anti spectrin  (Developmental Studies Hybridoma Bank)

Journal: bioRxiv

Article Title: Selective regulation of transsynaptic alignment and postsynaptic assembly by a novel NCAM family synaptic adhesion molecule

doi: 10.64898/2026.03.13.710942

Figure Lengend Snippet: Elff acts transsynaptically to promote alpha-Spectrin accumulation. (A-D) Representative Z-projections of boutons from the indicated genotypes stained for alpha-Spectrin (green) and HRP (magenta). Scale bar: 5 µm. (E) Quantification of alpha-Spectrin intensity. Data are mean values normalized to control ± SD (control: 100.0 ± 18.8, elff JR1/ST1 : 23.1 ± 4.5; control: 100.0 ± 16.0, Pre > elff RNAi #1: 50.8 ± 18.4, Pre > elff RNAi #2: 57.0 ± 28.5; control: 100.0 ± 17.0, Post > elff RNAi #1: 94.1 ± 12.4, Post > elff RNAi #2: 107.8 ± 19.8). (F-G) Representative Z-projections of muscle 4 NMJs of the indicated genotypes stained for alpha-Spectrin (green) and HRP (magenta). Arrows indicate synaptic boutons. Scale bars: 5 µm. (H) Orthogonal projections from synaptic boutons (left) and expanded HRP webbing (right) in Post > Elff. Dotted lines in G indicate the areas used to generate orthogonal projections. Scale bars: 5 µm. (I) Quantification of NMJ webbing phenotype (control: 0% of NMJs with webbing, Pre > Elff: 5.50% of NMJs with webbing, Post > Elff: 71.0% of NMJs with webbing). Significance determined by Kruskal-Wallis test with Dunn’s multiple comparisons (E) or chi-square test (I) [ns, not significant, ****p<0.0001]. n ≥ 27, Animals ≥ 8.

Article Snippet: The following commercially available primary antibodies were used: anti-Bruchpilot (DSHB, nc82) [1:100]; anti-Discs large (DSHB, 4F3) [1:500 third instar, 1:100 second instar, 1:100 embryo]; anti-alpha Spectrin (DSHB, 3A9) [1:100 third instar, 1:50 second instar].

Techniques: Staining, Control

Journal: bioRxiv

Article Title: Selective regulation of transsynaptic alignment and postsynaptic assembly by a novel NCAM family synaptic adhesion molecule

doi: 10.64898/2026.03.13.710942

Figure Lengend Snippet: Elff is required for the differentiation of the postsynaptic compartment during mid-larval development. (A-F) Representative Z-projections of boutons in second instar larvae of the indicated genotypes, stained for HRP (magenta) and (A-B) alpha-Spectrin, (C-D) Dlg, or (E-F) GluRIIE (grayscale). Scale bar: 2 µm. (G) Quantification of alpha-Spectrin intensity. Data are mean values normalized to control ± SD (control: 100 ± 48.80, elff JR1/ST1 : 6.665 ± 8.820). (H) Quantification of Dlg intensity (control: 100 ± 48.42, elff JR1/ST1 : 10.81 ± 10.57). (I) Quantification of GluRIIE intensity (control: 100 ± 29.30, elff JR1/ST1 : 5.311 ± 5.195). Significance determined by Mann-Whitney test [****p<0.0001]. n ≥ 20, Animals ≥ 5.

Article Snippet: The following commercially available primary antibodies were used: anti-Bruchpilot (DSHB, nc82) [1:100]; anti-Discs large (DSHB, 4F3) [1:500 third instar, 1:100 second instar, 1:100 embryo]; anti-alpha Spectrin (DSHB, 3A9) [1:100 third instar, 1:50 second instar].

Techniques: Staining, Control, MANN-WHITNEY

Journal: bioRxiv

Article Title: Selective regulation of transsynaptic alignment and postsynaptic assembly by a novel NCAM family synaptic adhesion molecule

doi: 10.64898/2026.03.13.710942

Figure Lengend Snippet: A model of distinct mechanisms for Elff’s functions in postsynaptic maturation and glutamate receptor clustering. Left: Elff is required in trans for postsynaptic accumulation of spectrin. Its postsynaptic partner(s) and other possible interactors are not known. Right: Elff facilitates glutamate receptor clustering and synaptic apposition in cis and/or in trans by stabilizing transsynaptic interactions through an unknown mechanism. In this context, Elff may act as a membrane-bound protein and/or as a secreted adaptor for other adhesion proteins in the extracellular matrix of the synaptic cleft.

Article Snippet: The following commercially available primary antibodies were used: anti-Bruchpilot (DSHB, nc82) [1:100]; anti-Discs large (DSHB, 4F3) [1:500 third instar, 1:100 second instar, 1:100 embryo]; anti-alpha Spectrin (DSHB, 3A9) [1:100 third instar, 1:50 second instar].

Techniques: Membrane